Yes, but you will not be able to dock them and use the remote of the unit. 2 possible options exist. * Option 1: If your MP3 player does not support DRM, you may select to hook it to the USB Port. Press SOURCE once or more (or USB/CARD on the remote control) to select USB/Card. * Option 2: Use the 3.5mm cable supplied with the device and hook the headphone jack of your MP3 Player to the Aux input on top of the unit. Press SOURCE once or more (or AUX on the remote control) to select AUX.